Proteína inhibidora de la poligalacturonasa en manzana mexicana (Malus domestica Borkh)
Mexican apple (Malus domestica Borkh) polygalacturonase inhibitor protein
Resumen
La poligalacturonasa (PG) es una de las enzimas responsables de la pérdida de firmeza de la manzana. La determinación de la presencia y actividad de la proteína inhibidora de la poligalacturonasa (PIPG) es información de alta relevancia como base para otros trabajos, como los relacionados a la incidencia y severidad de enfermedades fungosas en poscosecha, como información básica para su control. Con esta finalidad, se determinó la presencia y actividad de la PIPG en frutos de manzana por medio de un análisis de difusión radial, determinando también índices de madurez y contenido de proteínas totales. Los 20 frutos de cada cultivar, provenientes de una huerta comercial en Cuauhtémoc, Chihuahua, se cosecharon por fecha, pizcando al azar, bajo un diseño experimental completamente al azar. ABSTRACT Polygalacturonase (PG) is one of the enzymes responsible for apple fruit firmness loss, therefore, the determination of the presence and activity of polygalacturonase inhibitor protein (PGIP) is highly relevant information for those studies related to incidence and severity of postharvest fungal diseases and their control. PGIP presence and activity were determined on apple fruit by a radial diffusion analysis, maturity indexes and total protein content were also quantified. Twenty apple fruits of each cultivar were sampled from a commercial orchard in Cuauhtemoc, Chihuahua, Mexico. Apple fruits were sampled by date, picking them at random, under a completely randomized experimental design.Citas
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