@article{Hernández-Quezada_Arévalo-Gallegos_Aguado-Santacruz_Betancourt-Guerra_Siquieros-Cendón_Rivera-Chavira_Nevárez-Moorillón_Rascón-Cruz_2020, title={Método para la extracción de ADN cloroplastídico de Bouteloua gracilis como herramienta para aplicaciones moleculares : Method of chloroplast DNA extraction from Bouteloua gracilis as a tool for molecular applications }, volume={5}, url={https://vocero.uach.mx/index.php/tecnociencia/article/view/691}, DOI={10.54167/tch.v5i3.691}, abstractNote={<div class="stl_01"><span class="stl_17">La manipulación genética del genoma de cloroplasto esta a la </span><span class="stl_17 stl_08">vanguardia en investigación biotecnológica. El pasto</span><span class="stl_17 stl_08">&nbsp;</span><span class="stl_18 stl_08">B.</span><span class="stl_18 stl_08">&nbsp;gracilis</span><span class="stl_18 stl_08">&nbsp;</span><span class="stl_17 stl_08">es &nbsp;</span><span class="stl_17 stl_15">un modelo para el estudio de estrés hídrico y oxidativo, capacidades </span><span class="stl_17 stl_15">relacionadas al cloroplasto. Es necesario tener un método que facilite &nbsp;</span><span class="stl_17 stl_15">la obtención de</span><span class="stl_17 stl_15">&nbsp;ADN</span><span class="stl_17 stl_15">&nbsp;de buena calidad, particularmente cuando</span><span class="stl_17 stl_15"> se </span><span class="stl_17 stl_15">refiere al</span><span class="stl_17 stl_15">&nbsp;ADN</span><span class="stl_17 stl_15">&nbsp;de cloroplasto de</span><span class="stl_17 stl_15">&nbsp;</span><span class="stl_18 stl_15">B.</span><span class="stl_18 stl_15">&nbsp;gracilis</span><span class="stl_17 stl_15">. La implementación</span><span class="stl_17 stl_15">&nbsp;de &nbsp;</span><span class="stl_17 stl_15">un método para la extracción de cpADN se logró probando diferentes &nbsp;</span><span class="stl_17 stl_08">estrategias de extracción de cpADN reportados en la literatura y &nbsp;</span><span class="stl_17 stl_22">combinando las etapas más apropiadas para</span><span class="stl_17 stl_22">&nbsp;</span><span class="stl_18 stl_22">B.</span><span class="stl_18 stl_22">&nbsp;gracilis</span><span class="stl_17 stl_22">.</span><span class="stl_17 stl_22">&nbsp;La &nbsp;</span><span class="stl_17 stl_15">incorporación de un paso adicional del lavado con CTAB permitió la &nbsp;</span></div> <div class="stl_01"><span class="stl_17 stl_15">recuperación de cpDNA enriquecido, el cual se puede utilizar en el </span><span class="stl_17 stl_15">desarrollo de hermanitas molecular<span class="stl_08">es. Debido la implementación de &nbsp;</span></span><span class="stl_17 stl_15">un protocolo de extracción también fue posible obtener una cantidad &nbsp;</span><span class="stl_17 stl_15">considerable de cpADN de</span><span class="stl_17 stl_15">&nbsp;</span><span class="stl_18 stl_08">B.</span><span class="stl_18 stl_15">&nbsp;gracilis.</span><span class="stl_18 stl_15">&nbsp;</span><span class="stl_17 stl_15">El cpADN fue digerido</span><span class="stl_17 stl_15">&nbsp;con &nbsp;</span><span class="stl_17 stl_15">varias enzimas de restricción y los fragmentos resultantes fueron </span><span class="stl_17 stl_15">analizados por Southern blot con las sondas de los genes de rADN 16S y 23S.</span></div> <div class="stl_01"> <div class="stl_01"><strong><span class="stl_07 stl_08">Abstract &nbsp;</span></strong></div> <div class="stl_01"><span class="stl_17 stl_08">Genetic manipulation of the chloroplast genome is at the forefront &nbsp;</span><span class="stl_17 stl_08">of biotechnology research.</span><span class="stl_17 stl_08">&nbsp;</span><span class="stl_18 stl_08">B. gracilis</span><span class="stl_18 stl_08">&nbsp;</span><span class="stl_17">is</span><span class="stl_17 stl_08">&nbsp;a model for the</span><span class="stl_17 stl_08">&nbsp;study &nbsp;</span><span class="stl_17 stl_19">of water stress and oxidative capacities related to the &nbsp;</span><span class="stl_17 stl_08">chloroplast. It is requires a method to facilitate the obtaining of &nbsp;</span><span class="stl_17 stl_20">good quality DNA, particularly when it comes to chloroplast &nbsp;</span></div> <div class="stl_01"><span class="stl_17 stl_08">DNA of&nbsp;</span><span class="stl_18 stl_08">B. gracilis</span><span class="stl_17 stl_08">. The implementation of a method for extracting &nbsp;</span><span class="stl_17">cpDNA was achieved by assaying different strategies cpDNA &nbsp;</span><span class="stl_17 stl_20">extraction reported in the literature and combining the most &nbsp;</span><span class="stl_17 stl_21">appropriate stage for</span><span class="stl_17 stl_21">&nbsp;</span><span class="stl_18 stl_21">B.</span><span class="stl_18 stl_21">&nbsp;gracilis</span><span class="stl_18 stl_21">&nbsp;</span><span class="stl_17 stl_21">for</span><span class="stl_17 stl_21">&nbsp;cpDNA extraction.</span><span class="stl_17 stl_21">&nbsp;The &nbsp;</span><span class="stl_17 stl_08">incorporation of an additional step of washing with CTAB after &nbsp;</span><span class="stl_17 stl_08">lysis of chloroplasts, allowed the recovery of enriched cpDNA </span><span class="stl_17 stl_08">which can be used in the development of molecular tools. Due &nbsp;</span><span class="stl_17 stl_08">to the implementation of an extraction protocol was also possible &nbsp;</span></div> <div class="stl_01"><span class="stl_17 stl_08">to obtain a considerable amount of&nbsp;</span><span class="stl_18 stl_08">B. gracilis&nbsp;</span><span class="stl_17 stl_08">cpDNA. The cpDNA &nbsp;</span><span class="stl_17 stl_08">was digested with several restriction enzymes and the resulting&nbsp; f</span><span class="stl_17 stl_08">ragments were analyzed by Southern b<span class="stl_10">lot with probes of genes <span class="stl_17 stl_15">rADN 16S and 23S.</span></span></span></div> </div&gt;}, number={3}, journal={TECNOCIENCIA Chihuahua}, author={Hernández-Quezada, David I. and Arévalo-Gallegos, Sigifredo and Aguado-Santacruz, Armando and Betancourt-Guerra, David A. and Siquieros-Cendón, Tania and Rivera-Chavira, Blanca and Nevárez-Moorillón, Guadalupe Virginia and Rascón-Cruz, Quintín}, year={2020}, month={nov.}, pages={132-139} }