Cypermethrin, deltamethrin and glyphosate affect the activity of the Ca2 +-ATPase from human erythrocyte
Cipermetrina, deltametrina y glifosato afectan la actividad enzimática de la Ca2+-ATPasa de eritrocito humano
Resumen
Abstract
The extensive use of pesticides can cause many human health problems. However, the effects of pesticides on a biochemical level are still poorly understood. In this study we analyzed the effect of different pesticides on the plasma membrane Ca2+-ATPase (PMCA) and on the sarco/ endoplasmic reticulum Ca2+-ATPase (SERCA) activities. Different amounts of pesticides were added to the Ca2+- dependent ATPase assays in order to determine if they were affecting the ATPase activity. The results showed that PMCA activity was partially inhibited by deltamethrin to 51.85% ± 3.7 when its concentration in the reaction medium was 0.5 mM, while cypermethrin and glyphosate stimulated the PMCA activity at the lowest concentrations tested. The maximum stimulatory effect of cypermethrin was of 155% ± 9.0 at a concentration of 0.2 mM. In addition, the herbicide glyphosate stimulated the activity 111% ± 2.0 at a concentration of 0.2 mM. In conclusion, our results showed that PMCA activity was partially inhibited by deltamethrin, but cypermethrin and glyphosate stimulated their activity. Our findings suggest that cypermethrin and deltamethrin have different structure- activity relationships. However, SERCA was not sensitive to deltamethrin or glyphosate. These differences may be reflected in disturbs over cellular calcium regulation.
Resumen
Se ha observado que la exposición de personas a plaguicidas puede causar problemas a la salud. Sin embargo, el estudio del efecto de plaguicidas a un nivel bioquímico ha sido pobremente estudiado. En este estudio, analizamos el efecto de cipermetrina, deltametrina y glifosato sobre la actividad enzimática de las Ca2+- ATPasa de membrana plasmática (PMCA) y de retículo sarcoplásmico (SERCA). Diferentes concentraciones de estos pesticidas fueron añadidas a los experimentos de actividad enzimática como estrategia para determinar si estos compuestos eran capaces de afectar la actividad enzimática de PMCA. Nuestros resultados demuestran que la actividad enzimática de PMCA fue parcialmente inhibida por deltametrina en un 51.85% ± 3.7 cuando su concentración fue de 0.5 mM, mientras que cipermetrina y glifosato estimularon la actividad enzimática de PMCA a menores concentraciones. El máximo efecto estimulatorio de cipermetrina fue de 155% ± 9.0 cuando el compuesto alcanzó una concentración de 0.2 mM. Además, el herbicida glifosato fue capaz de estimular la actividad enzimática de PMCA en un 111% ± 2.0 a concentraciones de 0.1-0.2 mM. En conclusión, nuestros resultados demostraron que la actividad enzimática de PMCA fue también parcialmente inhibida con deltametrina, pero al contrario de cipermetrina y glifosato la estimularon. Nuestros resultados sugieren que las diferencias en las estructuras químicas de cipermetrina y deltametrina se ven reflejadas en el efecto provocado sobre PMCA. Sin embargo, una enzima similar, SERCA, no fue afectada ni por deltametrina ni glifosato. Estas diferencias se pudieran ver reflejadas en disturbios sobre la regulación celular del calcio.
Palabras clave: PMCA (ATPasa de Ca2+ de membrana plasmática), SERCA (ATPasa de Ca2+ de retículo endo/sarcoplásmico), actividad enzimática, cipermetrina, deltametrina, glifosato.
Citas
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