Tasa de fertilización, desarrollo y calidad de embriones bovinos Holstein producidos in vitro con semen sexado y adición de IGF-I
Fertilization rate, development and quality of Holstein bovine embryos produced in vitro with sexed semen and addition of IGF-I
Resumen
La adición de IGF-I a los medios de fertilización in vitro y cultivo de embriones ha sido propuesta como una forma de imitar las señales maternas de la gestación temprana en el útero. El objetivo del presente estudio fue evaluar la adición del factor de crecimiento similar a la insulina tipo-I (IGF-I) sobre la tasa de fertilización con semen sexado (SS) y de blastocistos bovinos producidos in vitro, así como la calidad embrionaria. Se utilizó SS de tres toros Holstein para la fertilización in vitro (FIV) de ovocitos obtenidos de ovarios recolectados en rastro, para lo cual se agregó IGF-I (100 ng/ml) en los medios de fertilización y desarrollo embrionario a diferentes tiempos: T1 (IGF-I, d 0-7; n= 393); T2 (IGF-I, d 0-3; n= 394); T3 (IGF-I, d 3-7; n= 394); y T4 (sin IGF-I o grupo control; n= 394). Se evaluó el porcentaje de fertilización al día tres de incubación y el día siete se evaluó la calidad y estadio de los blastocistos. Los datos se analizaron con el procedimiento CATMOD de SAS (SAS Inst. Inc., Cary, NC), ajustando un modelo que incluyó el efecto de tratamiento (con y sin IGF-I, para tasa de fertilización; y T1, T2, T3 y T4 para otras variables). La adición de IGF-I al medio afectó la tasa de fertilización (34 vs 42%; P < 0.05) y no hubo efecto (P > 0.05) de tratamiento para la tasa de blastocistos/ovocitos fertilizados, ni para calidad de los blastocistos. Se concluye que bajo las condiciones del presente estudio, la adición de IGF-I a los medios de fertilización y cultivo in vitro no tiene un efecto benéfico para la producción de embriones, pero sí afecta la fertilidad del semen sexado.
Abstract
The addition of IGF-I to the in vitro fertilization and embryo culture media has been suggested as a means to imitate maternal signals during early gestation in the uterus. The aim of the present study was to evaluate the addition of insulin-like growth factor I (IGF- I) on the fertilization rate with sexed semen (SS) and bovine blastocysts produced in vitro as well as the embryo quality. Semen used was from three Holstein bulls for in vitro fertilization and oocytes were obtained from ovaries collected on a slaughterhouse. For which IGF-I (100 ng/ml) was added in the means of fertilization and embryo development at different times: T1 (IGF-I, d 0-7; n= 393); T2 (IGF-I, d 0-3; n= 394); T3 (IGF-I, d 3- 7; n= 394); and T4 (without IGF-I or control group; n= 394). Fertilization rate was evaluated at day 3 of incubation, and at day 7, blastocysts quality and stage were assessed. Data were analyzed with CATMOD of SAS (SAS Inst. Inc., Cary, NC), adjusting a model including the effect of treatment (with and without IGF-I, for fertilization rate; and T1, T2, T3 and T4, for other variables). The addition of IGF-I to the media affected the fertilization rate (34 vs 42%; P < 0.05) and there was not effect (P > 0.05) of treatment for the rate blastocysts/fertilized oocytes fertilized or for the quality of blastocysts. It is concluded that under the conditions of this study, with the use of sexed semen, the addition of IGF-I to the media of fertilization and culture in vitro does not have a beneficial effect on the production of embryos, but it does affect the fertility of the sexed semen.
Keywords: IGF-I, embryo, in vitro fertilization, sexed semen.
Citas
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