Cuantificación de los diferentes folatos presentes en tomatillo (Physalis ixocarpa) por cromatología de líquidos de alta resolución

Quantification of folates in Physalis ixocarpa by high pressure liquid chromatography

  • José Luis Ibave-González UACH. Facultad de Ciencias Agrotecnológicas
Palabras clave: tomatillo, folatos, HPLC, Physalis ixocarpa

Resumen

En este estudio se identificaron y determinaron las diferentes especies de folatos por medio de cromatografía liquida de alta resolución en fase reversa, con detección de fluorescencia, aunado a un tratamiento trienzimático. La deconjugación de las cadenas de glutamatos en los folatos se realizó mediante una columna de afinidad a folatos. La cantidad promedio de folatos fue de 1.2 μM/100 gramos de peso fresco. La composición de las principales estructuras de folatos presentes en Physalis correspondió a 5-metil- tetrahidrofolato, tetrahidrofolato y 5-formil-tetrahidrofolato. La valoración de estos importantes metabolitos dentro de la dieta se ha soslayado debido a la dificultad implícita en su análisis. Es evidente que la falta de esta vitamina está implicada en el riesgo de defectos del tubo neural y anencefalia, principalmente en embarazos no deseados, donde no se consume una dieta adecuada de suplementos antes de la concepción. Este problema puede ser disminuido con la ingesta de fuentes de folatos en la dieta como Physalis ixocarpa.

DOI: https://doi.org/10.54167/tecnociencia.v1i1.31

 

Citas

Berry, R.J., Z. Li, J. D. Erickson, S. Li, C.A. Moore, H. Wang, J. Mulinare, P. Zhao, L.Y. C. Wong, J. Gindler, S.X. Hong, L. Hao, E. Gunter & A. Correa. 1999. Prevention of neural-tube defects with folic acid in China. N. Engl. J. Med. 341:1485-1490. http://dx.doi.org/10.1056/NEJM199911113412001

Birktoft, J.J. & K. Bredman. 1994. Glutamyl endopeptidases. Methods in Enzymology 244:114-126. https://doi.org/10.1016/0076-6879(94)44010-7

Boushey, C.J., S.A.A. Beresford, G.S. Omenn & A.G. Motulsky. 1995. A quantitative assessment of plasma homocysteine as a risk factor for vascular disease. Probable benefits of increasing folic acid intakes. JAMA. 274(13):1049-1057. https://doi.org/10.1001/jama.1995.03530130055028

Černá, L. & J. Káš. 1983. New conception of folacin assay in starch or glycogen containing food samples. Nahrung 27(10):957-964. https://doi.org/10.1002/food.19830271013

Czeizel, A. & I. Dudás. 1992. Prevention of the first occurrence of neural tube defects by periconceptional vitamin supplementation. N. Engl. J. Med. 327(26):1832- 1835. https://doi.org/10.1056/nejm199212243272602

Díaz, R., E.P. Quinlivan, S. M.J. Klaus, G. J.C. Basset, J.F. Gregory III & A.D. Hanson. 2004. Folate biofortification in tomatoes by engineering the pteridine branch of folate synthesis. Proc. Nat. Ac. Sci. 101 (38):13720-13725. https://doi.org/10.1073/pnas.0404208101

Fohr, I.P., R. Prinz-Langenohl, A. Brönstrup, A. M. Bohlmann, H. Nau, H. K. Berthold & K. Pietrzik. 2002. 5,10 Methylenetetrahydrofolate reductase genotype determines the plasma homocysteine-lowering effect of supplementation with 5-methyltetrahydrofolate or folic acid in healthy young woman. Am. J. Clin. Nut. 75(2):275-282. https://doi.org/10.1093/ajcn/75.2.275

Giovannucci, E., E.B. Rimm, A. Ascherio, M.J. Stampfer, G.A. Colditz & W.C. Willett. 1995. Alcohol, low-methionine-lowfolate diets, and risk of colon cancer in men. J. Nat. Cancer Inst. 87(4):265-273. https://doi.org/10.1093/jnci/87.4.265

Gregory III, J.F., R. Engelhardt, S.D. Bhandari, D.B. Sartain & S.K. Gustafson.1990. Adequacy of extraction techniques for determination of folate in foods and other biological materials. J. Food Comp. An. 3(2):134- 144. https://doi.org/10.1016/0889-1575(90)90020-M

Gregory III, J.F., D.B. Sartain & B.P.F. Day. 1984. Fluorometric determination of folacin in biological materials using higher performance chromatography. J. Nutrition. 114(2):341–353. https://doi.org/10.1093/jn/114.2.341

Gregory III, J.F. & J.P. Toth. 1988. Chemical synthesis of deuterated folate monoglutamate and in vivo assessment of urinary excretion of deuterated folates in man. Anal. Biochem. 170(1):94-104. https://doi.org/10.1016/0003-2697(88)90094-2

Jastrebova, J., C. Witthöft, A. Grahn, U. Svensson & M. Jägerstad. 2003. HPLC determination of folates in raw and processed beetroots. Food Chem. 80 (4):579-588. https://doi.org/10.1016/S0308-8146(02)00506-X

Lucock, M.D., M. Green, M. Priestnall, I. Daskalakis, M.I. Levene & R. Hartley. 1995. Optimization of chromatographic conditions for the determination of folates in foods and biological tissues for nutritional and clinical work. Food Chem. 53(3):329-338. https://doi.org/10.1016/0308-8146(95)93941-J

Martin, J.I., W.O. Landen Jr., A.G. Soliman & R.R. Eitenmiller. 1990. Application of a tri-enzyme extraction for total folate determination in foods. J. Ass. Off. Anal. Chem. 73(5):805-808. https://pubmed.ncbi.nlm.nih.gov/2125600/

Mason, J. 1995. Folate status: effects on carcinogenesis. pp. 329-361. In: Bailey LB, ed., Folate in Health and Disease. New York: Marcel Dekker, Inc.

Nehmatallah, N.J. 2003. Capillary electrophoresis and high performance liquid choromatography determination of polyglutamyl folates in citrus products (Tesis, University of Florida). https://ufdcimages.uflib.ufl.edu/UF/E0/00/10/91/00001/UFE0001091.pdf

Peña, L.A. 2001. Situación actual y perspectivas de la producción y mejoramiento genético de tomate de cáscara (Physalis ixocarpa Brot.) en México. En Memoria del XXIV Congreso Nacional de Fitogenética. 4- 9 de octubre. UAAN. ISBN 9786078029068

Rader, J.I., C.M. Weaver & G. Angyal. 1998. Use of a micro-biological assay with trienzyme extraction for measurement of prefortification levels of folates in enriched cereal-grain products. Food Chem. 62(4):451-465. https://doi.org/10.1016/S0308-8146(98)00089-2

Ravanel, S., H. Cherest, S. Jabrin, D. Grunwald, Y. Surdin- Kerjan, R. Douce & F. Rébeillé. 2001. Tetrahydrofolate biosynthesis in plants: Molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana. Proc. Nat. Ac. Sci. 98(26):15360-15365. https://doi.org/10.1073/pnas.261585098

Robinson, D.R. 1971. The nonenzymatic hydrolysis of N5 N10-methenyltetrahydrofolic acid and related reactions. Methods Enzymol. 18(Part B):716-725. https://doi.org/10.1016/S0076-6879(71)18141-4

Sauberlich, H.E. 1995. Folate status of U. S. population groups. pp 171-194. In: Bailey LB, ed., Folate in Health and Disease. New York: Marcel Dekker, Inc.

Scholl, T.O. & W.G. Johnson. 2000. Folic Acid: influence on the outcome of pregnancy. Am. J. Clin. Nut. 71(5):1295- 1303. https://doi.org/10.1093/ajcn/71.5.1295s

Selhub, J., O. Ahmad & I.H. Rosenberg. 1980. Preparation and use of affinity columns with bovine milk folate-binding protein covalently linked to Sepharose 4B. Methods Enzymol. 66:686-690. https://doi.org/10.1016/0076-6879(80)66528-8

Tamura, T., Y. Mizuno, K.E. Johnson & R.A. Jacob. 1997. Food folate assay with protease a-amylase and folate conjugase treatments. J. Agr. Food Chem. 45(1):135-139. https://doi.org/10.1021/jf960497p

Tamura, T. 1998. Determination of food folate. Nutr. Biochem. 9(5): 285-293. https://doi.org/10.1016/S0955-2863(98)00013-8

Van Oort, F. V.A., A. Melse-Boonstra, I. A. Brower, R. Clarke, C. E. West, M. B. Katan & P. Verhoef. 2003. Folic acid and reduction of plasma homocysteine concentration in older adults, a dose response study. Am. J. Clin. Nut. 77 (5):1318-1323. https://doi.org/10.1093/ajcn/77.5.1318

Vahteristo, L.T., V. Ollilainen, P.E. Koivistoinen & P. Varo. 1996. Improvements in the analysis of reduced folate monoglutamates and folic acid in food by high-perfor- mance liquid chromatography. J. Agr. Food Chem. 44(2):477–482. https://doi.org/10.1021/jf9503467

Wagner, C. 1995. Biochemical role of folate in cellular metabolism. pp.23-42. In: Bailey LB, ed., Folate in Health and Disease. New York: Marcel Dekker, Inc.

Publicado
2007-01-08
Cómo citar
Ibave González, J. L. (2007). Cuantificación de los diferentes folatos presentes en tomatillo (Physalis ixocarpa) por cromatología de líquidos de alta resolución: Quantification of folates in Physalis ixocarpa by high pressure liquid chromatography. TECNOCIENCIA Chihuahua, 1(1), 9-16. https://doi.org/10.54167/tecnociencia.v1i1.31